While I locktite everything else on the bike, this is one area that does not get any locktite. Unlike other bolts that vibrate loose, these little nuts seem to stay firmly in place. Even with racing, and the hundreds of miles I put on my bike each month, I have never lost a nut. Also, I don't really tighten the nuts as one might expect, as I only use a 10mm T-handle. Fortunately, these little guys are easy to remove when the unfortunate happens.
As a note, on the only use bike I ever purchase back in the early 1990's, the prior owner had gotten a little excited while tightening the nuts, and had broken one stud. No knowing any better at the time, I rode the bike for a year and a half with 3 studs on one side, and never had a problem. Now, I am not recommending running 3 studs as a general rule, but I would certainly entertain continued riding for the day if I did happen to twist off a stud while changing a tire at the van. Wouldn't even give it a second thought.
Good luck with the removal.
Rick